Reaction: CYP51A1 demethylates LNSOL
- in pathway: Cholesterol biosynthesis
Lanosterol 14-alpha demethylase (CYP51A1) catalyses oxidative C14-demethylation of lanosterol (LNSOL) to 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol (4,4DMCHOLtrienol). Although the reaction is annotated here as a single concerted event, studies with purified rat enzyme indicate that the methyl group is converted successively to an alcohol and an aldehyde before being released as formate (Stromstedt et al. 1996, Strushkevich et al. 2010).
Reaction - small molecule participants:
H2O [cytosol]
NADP+ [cytosol]
4,4DMCHtOL [endoplasmic reticulum membrane]
HCOOH [cytosol]
H+ [cytosol]
O2 [cytosol]
LAN [endoplasmic reticulum membrane]
NADPH [cytosol]
H2O [cytosol]
NADP+ [cytosol]
4,4DMCHtOL [endoplasmic reticulum membrane]
HCOOH [cytosol]
H+ [cytosol]
O2 [cytosol]
LAN [endoplasmic reticulum membrane]
NADPH [cytosol]
Reactome.org reaction link: R-HSA-194678
======
Reaction input - small molecules:
hydron
dioxygen
lanosterol
NADPH(4-)
hydron
dioxygen
lanosterol
NADPH(4-)
Reaction output - small molecules:
water
NADP(3-)
4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol
formate
water
NADP(3-)
4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol
formate
Reactome.org link: R-HSA-194678