Reaction: CYP2R1 25-hydroxylates VD3 to 25(OH)D
- in pathway: Vitamin D (calciferol) metabolism
To be functionally active, vitamin D3 (VD3) needs to be dihydroxylated. The first hydroxylation at position 25 is carried out by ER membrane-located vitamin D 25-hydroxylase (CYP2R1) in the liver, forming 25-hydroxyvitamin D (calcidiol, 25(OH)D) (Shinkyo et al. 2004, Cheng et al. 2003).
Reaction - small molecule participants:
25(OH)D [endoplasmic reticulum membrane]
H2O [cytosol]
NADP+ [cytosol]
H+ [cytosol]
O2 [cytosol]
VD3 [endoplasmic reticulum membrane]
NADPH [cytosol]
25(OH)D [endoplasmic reticulum membrane]
H2O [cytosol]
NADP+ [cytosol]
H+ [cytosol]
O2 [cytosol]
VD3 [endoplasmic reticulum membrane]
NADPH [cytosol]
Reactome.org reaction link: R-HSA-209845
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Reaction input - small molecules:
hydron
dioxygen
calciol
NADPH(4-)
hydron
dioxygen
calciol
NADPH(4-)
Reaction output - small molecules:
calcidiol
water
NADP(3-)
calcidiol
water
NADP(3-)
Reactome.org link: R-HSA-209845