Reaction: GAR + 10-Formyl-THF => FGAR + THF
- in pathway: Purine ribonucleoside monophosphate biosynthesis
The irreversible transfer of a formyl group to cytosolic 5-phosphoribosylglycinamide (GAR) to form 5'-phosphoribosylformylglycinamide (FGAR) is catalyzed by the phosphoribosylglycinamide formyltransferase domain of the trifunctional protein, "phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase" (GART) (Aimi et al. 1990; Zhang et al. 2002). Fluoresence microscopy studies of cultured human cells have shown that GART is cytosolic and suggest that it may co-localize with other enzymes of de novo IMP biosynthesis under some metabolic conditions (Gooljarsingh et al. 2001; An et al. 2008).
Reaction - small molecule participants:
H+ [cytosol]
N-Formyl-GAR [cytosol]
THF [cytosol]
PRGA [cytosol]
10-formyl-THF [cytosol]
Reactome.org reaction link: R-HSA-73813
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Reaction input - small molecules:
N(1)-(5-phospho-beta-D-ribosyl)glycinamide(1-)
10-formyltetrahydrofolate(2-)
Reaction output - small molecules:
hydron
N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide(2-)
(6S)-5,6,7,8-tetrahydrofolate(2-)
Reactome.org link: R-HSA-73813