Reaction: 5,10-methyleneTHF polyglutamate + NADP+ <=> 5,10-methenylTHF polyglutamate + NADPH + H+
- in pathway: Metabolism of folate and pterines
The methylenetetrahydrofolate dehydrogenase activity of the trifunctional MTHFD1 enzyme catalyzes the reversible reaction of 5,10-methyleneTHF polyglutamate and NADP+ to form 5,10-methenylTHF polyglutamate, NADPH, and H+. MTHFD1 is cytosolic and occurs as a dimer. The human enzyme has been identified and partially characterized biochemically (Hum et al. 1988); additional reaction details can be inferred from the properties of the well-studied homologous rabbit enzyme (Villar et al. 1985).
Reaction - small molecule participants:
H+ [cytosol]
NADPH [cytosol]
5,10-methenyl-THFPG [cytosol]
NADP+ [cytosol]
5,10-methylene-THFPG [cytosol]
Reactome.org reaction link: R-HSA-200644
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Reaction input - small molecules:
NADP(3-)
5,10-methylenetetrahydrofolate polyglutamate macromolecule
Reaction output - small molecules:
hydron
NADPH(4-)
5,10-methenyltetrahydrofolate polyglutamate macromolecule
Reactome.org link: R-HSA-200644